01390nas a2200241   4500000000100000008004100001260001700042653002400059653001200083100001600095700001500111700001300126700001400139700001200153700001400165700001400179700001100193245009400204300003300298490000700331050000600338520080400344       2005                            d  c12928489949010aPeer Reviewed Paper10aChecked1 aElkins Mark1 aHarbour C.1 aPunch G.1 aSyrmis M.1 aRose B.1 aNissen M.1 aSloots T.1 aBye P.00aMethod for detection of respiratory viruses in the sputa of patients with cystic fibrosis  a54-57. [Impact Factor 2.309]0 v24  aN3 a<p>
Since the role of respiratory viruses in lung exacerbations of patients with cystic fibrosis has been hampered by the difficulty of detecting viruses in viscous sputum specimens, a multiplex reverse transcriptase PCR (RT-PCR) assay combined with colorimetric amplicon detection was tested for the identification of seven common respiratory viruses in the sputa of cystic fibrosis patients. Of 52 sputa from 38 patients, 12 (23%) samples from 12 patients were positive for a respiratory virus (4 for influenza B, 3 for parainfluenza 1, 3 for influenza A and 2 for respiratory syncytial virus). These results suggest that the RT-PCR method carried out on sputum may provide a convenient means of investigating the role of virus infection in lung exacerbations of cystic fibrosis patients.
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